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[DOI] [PMC free article] [PubMed] [Google Scholar] 8.Yang I.A., Jenkins C.R., Salvi S.S

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The section was then incubated with recombinant anti-mucin 5AC antibody (ab3649, Abcam) for 1 h at room temperature, followed by biotinylated secondary antibody (Dako) and HRP detection (HRP conjugated ABC system, Vector Laboratories), according to manufacturers instruction

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The upregulation and activation of 7nAChR subsequently activate several signaling pathways, including neuroprotective PI3K/Akt and Nrf2 pathways, then suppression of pro-apoptotic caspase-3, and suppression of NF-B that reduce the expression of proinflammatory cytokines such as IL-1, and TNF- [50, 86]

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