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doi: 10.1111/vco.12309

The specific timing depends on your individual protocol

The levels of VEGF-A protein in serum-free culture supernatants (50 g/ml) from EBC-1 cells treated with or without siRNAs were measured using the Human VEGF-A ELISA Kit (RayBiotech, Inc.) according to the manufacturer's instructions as described previously (8)

In NSCLC, Alimta is approved for various settings, including initial treatment in combination with cisplatin for locally advanced or metastatic non-squamous NSCLC, as maintenance therapy after initial chemotherapy, and as a single agent for patients with locally advanced or metastatic non-squamous NSCLC after prior chemotherapy

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