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Cloudiness, particles, color change, or a compromised stopper are discard signals, but a clear solution can still degrade chemically over time
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PMID: 36621481 Mater Today Adv2023, 17: 100336
Cai, H., Wang, C
After multiple washing steps, the incubation system retains only the bait protein immobilized on the resin along with the target proteins bound to it
