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The final pRMC2mKate plasmid was verified by restriction digest and Sanger sequencing, and then transformed into RN4220 and subsequently HG003

Rotate sites systematically (abdomen avoiding 2inch radius around navel, outer thighs, upper arms) to avoid lipohypertrophy [8]

Data acquisition was performed using version 7 of pClamp software (Axon Instruments)

(Use as per package insert)

Loss of core fucosylation of low-density lipoprotein receptor-related protein-1 impairs its function, leading to the upregulation of serum levels of insulin-like growth factor-binding protein 3 in Fut8 / mice