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Cells were disrupted by sonication and the lysate was clarified by centrifugation at 35,000 g for 60 min at 4 C, followed by filtration through a 0.45 m membrane

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Other variables, such as the drying method, washing duration, and the inherent variability of the mushroom samples, may also exert significant influence on the extraction rate

The study utilized HA with different molecular weightslow molecular weight (LMW) and high molecular weight (HMW)due to their presumed distinct biological activities

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