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The selectivity of NNMT inhibitors was confirmed by testing against a panel of structurally similar methyltransferases and two enzymes in the NAD+ salvage pathway (NAMPT and SIRT1;FIGS
Ponikowski, P
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The above procedure was repeated with inhibitor concentrations spanning 0.3 60 M to determine the effective concentration (EC 50 ) required to inhibit 50% NNMT activity in cultured adipocytes
