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Panc-1 cells, which originated from human pancreas where chymotrypsin is synthesized, were cultured in Dulbeccos Modified Eagle Medium (DMEM, Invitrogen, 11965092) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, A4736201) and penicillin-streptomycin (PS, Invitrogen, 15140122), collected, and lysed on ice with the chymotrypsin assay buffer provided by the kit

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