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10.1200/JCO.18.00313 J Clin Oncol
Methods: We use mathematical models for hepatic glutathione metabolism, including one-carbon metabolism and acetaminophen detoxification, to investigate how the activation of certain enzymes by estradiol leads to dramatic changes in reaction velocities and metabolite concentrations
Ultrathin sections (80 nm thick) were cut with an ultramicrotome (Leica, EMUC6, Germany), and then stained with uranyl acetate and lead citrate and finally examined by a Tecnai G 2 Spirit TWIN transmission electron microscope (FEI, Hillsboro, OR, USA)
We thank Danile Spehner from IGBMC, and Paolo Ronchi and Yannick Schwab from the Electron Microscopy Core Facility at the European Molecular Biology Laboratory Heidelberg for their help with electron microscopy
One of these regulating factors is insulin-like growth factor-1
Most people notice visible changes within 412 weeks
