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Briefly, for chromatographic separation of amino acids and acylcarnitines, a 2.1 100 mm, 100 , 1.7 m, UPLC BEH amide column (Waters, Eschborn, Germany) was used with 5 mM ammonium acetate in water (eluent A) and 5 mM ammonium acetate in acetonitrile/water (95/5, v/v) (eluent B) as elution solvents both adjusted to pH 3 using acetic acid

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Functional predictions were performed using PICRUST [63], Tax4Fun [64], and FAPROTAX [65]

Selenium decreases thyroid cancer cell growth by increasing expression of Gadd153 and Gadd34

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