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Primary antibodies were incubated at 4C overnight and secondary antibodies were applied for 90 minutes at room temperature and then developed with Supersignal West Dura (Thermo Fisher #34075) or Supersignal West Femto (Thermo Fisher #34096)

Clinical trials demonstrated 15-20% reduction in visceral adipose tissue over 26 weeks while subcutaneous fat remained largely unchanged

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Vitamin C inhibits FASinduced apoptosis in monocytes and U937 cells